import logging
from dataclasses import dataclass
from typing import Optional, Sequence, Tuple
import numpy as np
import zarr
from skimage.transform import rescale
from upath import UPath
from cellmap_data import CellMapImage
logger = logging.getLogger(__name__)
def _get_attr_any(attrs, keys):
for k in keys:
if k in attrs:
return attrs[k]
return None
def _parse_voxel_size(attrs) -> Optional[Tuple[float, ...]]:
vs = _get_attr_any(attrs, ["voxel_size", "resolution", "scale"])
if vs is None:
return None
return tuple(float(x) for x in vs)
def _parse_translation(attrs) -> Optional[Tuple[float, ...]]:
tr = _get_attr_any(attrs, ["translation", "offset"])
if tr is None:
return None
return tuple(float(x) for x in tr)
def _resize_pad_crop(
image: np.ndarray, target_shape: Tuple[int, ...], pad_value=0
) -> np.ndarray:
# center pad/crop like your resize_array
arr_shape = image.shape
resized = image
pad_width = []
for i in range(len(target_shape)):
if arr_shape[i] < target_shape[i]:
pad_before = (target_shape[i] - arr_shape[i]) // 2
pad_after = target_shape[i] - arr_shape[i] - pad_before
pad_width.append((pad_before, pad_after))
else:
pad_width.append((0, 0))
if any(p > 0 for pads in pad_width for p in pads):
resized = np.pad(resized, pad_width, mode="constant", constant_values=pad_value)
slices = []
for i in range(len(target_shape)):
if resized.shape[i] > target_shape[i]:
start = (resized.shape[i] - target_shape[i]) // 2
end = start + target_shape[i]
slices.append(slice(start, end))
else:
slices.append(slice(None))
return resized[tuple(slices)]
[docs]
@dataclass
class MatchedCrop:
path: str | UPath
class_label: str
target_voxel_size: Sequence[float]
target_shape: Sequence[int]
target_translation: Sequence[float]
instance_classes: Sequence[str]
semantic_threshold: float = 0.5
pad_value: float | int = 0
def _is_instance(self) -> bool:
return self.class_label in set(self.instance_classes)
def _select_non_ome_level(
self, grp: zarr.Group
) -> Tuple[str, Optional[Tuple[float, ...]], Optional[Tuple[float, ...]]]:
"""
Heuristic: choose among arrays like s0/s1/... (or any array keys) by voxel_size attrs.
Preference: pick the level whose voxel_size is <= target_voxel_size (not finer than target)
and closest to target; else closest overall.
Returns (array_key, voxel_size, translation)
"""
keys = list(grp.array_keys())
if not keys:
raise ValueError(f"No arrays found under {self.path}")
tgt = np.asarray(self.target_voxel_size, dtype=float)
best_key = None
best_vs = None
best_tr = None
best_score = None
for k in keys:
arr = grp[k]
vs = _parse_voxel_size(arr.attrs)
tr = _parse_translation(arr.attrs)
if vs is None:
# treat as unknown; deprioritize
score = 1e18
else:
v = np.asarray(vs, dtype=float)
if v.size != tgt.size:
v = v[-tgt.size :]
# prefer v >= tgt? (coarser) or v <= tgt? depends on definition.
# Here: voxel_size larger => coarser. We want not finer than target => v >= tgt
# If your convention is reversed, adjust this rule.
not_finer = np.all(v >= tgt)
dist = float(np.linalg.norm(v - tgt))
score = dist + (0.0 if not_finer else 1e6)
if best_score is None or score < best_score:
best_score = score
best_key = k
best_vs = vs
best_tr = tr
assert best_key is not None
return best_key, best_vs, best_tr
def _load_source_array(self):
"""
Returns (image, input_voxel_size, input_translation)
where image is a numpy array.
"""
ds = zarr.open(str(self.path), mode="r")
# OME-NGFF multiscale
if isinstance(ds, zarr.Group) and "multiscales" in ds.attrs:
img = CellMapImage(
path=str(self.path),
target_class=self.class_label,
target_scale=self.target_voxel_size,
target_voxel_shape=self.target_shape,
pad=True,
pad_value=self.pad_value,
interpolation="nearest" if self._is_instance() else "linear",
)
level = img.scale_level
level_path = UPath(self.path) / level
# Extract input voxel size and translation from multiscales metadata
input_voxel_size = None
input_translation = None
for d in ds.attrs["multiscales"][0]["datasets"]:
if d["path"] == level:
for t in d.get("coordinateTransformations", []):
if t.get("type") == "scale":
input_voxel_size = tuple(t["scale"])
elif t.get("type") == "translation":
input_translation = tuple(t["translation"])
break
arr = zarr.open(level_path.path, mode="r")
image = arr[:]
return image, input_voxel_size, input_translation
# Non-OME group multiscale OR single-scale array with attrs
if isinstance(ds, zarr.Group):
# If this group directly contains the label array (common): path points at an array node
# zarr.open on an array path usually returns an Array, not Group. If we got Group, pick a level.
key, vs, tr = self._select_non_ome_level(ds)
arr = ds[key]
image = arr[:]
return image, vs, tr
# Single-scale zarr array
if isinstance(ds, zarr.Array):
image = ds[:]
return image, _parse_voxel_size(ds.attrs), _parse_translation(ds.attrs)
raise ValueError(f"Unsupported zarr node type at {self.path}: {type(ds)}")
[docs]
def load_aligned(self) -> np.ndarray:
"""
Return full aligned volume in target space (target_shape).
"""
image, input_voxel_size, input_translation = self._load_source_array()
tgt_vs = np.asarray(self.target_voxel_size, dtype=float)
tgt_shape = tuple(int(x) for x in self.target_shape)
tgt_tr = np.asarray(self.target_translation, dtype=float)
# Resample if needed
if input_voxel_size is not None:
in_vs = np.asarray(input_voxel_size, dtype=float)
if in_vs.size != tgt_vs.size:
in_vs = in_vs[-tgt_vs.size :]
if not np.allclose(in_vs, tgt_vs):
scale_factors = in_vs / tgt_vs
if self._is_instance():
image = rescale(
image,
scale_factors,
order=0,
mode="constant",
preserve_range=True,
).astype(image.dtype)
else:
imgf = rescale(
image,
scale_factors,
order=1,
mode="constant",
preserve_range=True,
)
image = imgf > self.semantic_threshold
elif image.shape != tgt_shape:
# If no voxel size info, fall back to center crop/pad
image = _resize_pad_crop(image, tgt_shape, pad_value=self.pad_value)
# Compute relative offset in voxel units
if input_translation is not None:
in_tr = np.asarray(input_translation, dtype=float)
if in_tr.size != tgt_tr.size:
in_tr = in_tr[-tgt_tr.size :]
# snap to voxel grid
adjusted_in_tr = (in_tr // tgt_vs) * tgt_vs
rel = (np.abs(adjusted_in_tr - tgt_tr) // tgt_vs) * np.sign(
adjusted_in_tr - tgt_tr
)
rel = rel.astype(int)
else:
rel = np.zeros(len(tgt_shape), dtype=int)
# Translate + crop/pad into destination
if any(rel != 0) or image.shape != tgt_shape:
result = np.zeros(tgt_shape, dtype=image.dtype)
input_slices = []
output_slices = []
for d in range(len(tgt_shape)):
if rel[d] < 0:
input_start = abs(rel[d])
output_start = 0
input_end = min(input_start + tgt_shape[d], image.shape[d])
length = input_end - input_start
output_end = output_start + length
else:
input_start = 0
output_start = rel[d]
output_end = min(tgt_shape[d], image.shape[d] + output_start)
length = output_end - output_start
input_end = input_start + length
if length <= 0:
return result
input_slices.append(slice(int(input_start), int(input_end)))
output_slices.append(slice(int(output_start), int(output_end)))
result[tuple(output_slices)] = image[tuple(input_slices)]
return result
return image
